A modified yeast three-hybrid system enabling both positive and negative selections
نویسندگان
چکیده
منابع مشابه
Selections that optimize RNA display in the yeast three-hybrid system.
The yeast three-hybrid system (Y3H) is a powerful tool to select or confirm RNA-protein interactions. Target protein recognition of an RNA insert within a test transcript depends on at least three factors: intrinsic protein affinity for the properly folded insert, retention of RNA insert tertiary structure within a longer RNA transcript, and accessibility of the RNA insert to the target protein...
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We have used the yeast three-hybrid system in a positive selection for mutants of the human histone hairpin-binding protein (HBP) capable of interacting with non-canonical hairpins and in a negative selection for loss-of-binding mutants. Interestingly, all mutations from the positive selection are located in the N- and C-terminal regions flanking a minimal RNA-binding domain (RBD) previously de...
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RNA-protein interactions are essential for the proper execution and regulation of every step in the life of a eukaryotic mRNA. Here we describe a three-hybrid system in which RNA-protein interactions can be analyzed using simple phenotypic or enzymatic assays in Saccharomyces cerevisiae. The system can be used to detect or confirm an RNA-protein interaction, to analyze RNA-protein interactions ...
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Rats were required to press a bar to activate a motor-driven wheel that forced them to run and subsequently to drink to turn off the wheel. Barpressing and lickilng increased, showing the onset and offset of running to be positively and negatively reinforcing, respectively. The experimental control of the offset of running, in contrast to the traditional control for onset only, served to demons...
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ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2018
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-018-2567-7